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Western blot analysis in Jurkat cell line lysates (35ug/lane).
PLS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

PLS1 Antibody

CATALOG NUMBER: 56-881

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PLS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 550-579 amino acids from the C-terminal region of human PLS1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
70 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLS1
Additional Names:
Plastin-1, Intestine-specific plastin, I-plastin, PLS1
Protein Accession Number:
Q14651
Protein Gi Number:
224471848
Ncbi Gene Id Number:
5357
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Plastins are a family of actin-binding proteins that areconserved throughout eukaryote evolution and expressed in mosttissues of higher eukaryotes. In humans, two ubiquitous plastinisoforms (L and T) have been identified. The protein encoded bythis gene is a third distinct plastin isoform, which isspecifically expressed at high levels in the small intestine.Alternatively spliced transcript variants varying in the 5' UTR,but encoding the same protein, have been found for this gene. Apseudogene of this gene is found on chromosome 11.
Background Reference 1:
Chafel, M.M., et al. Dev. Dyn. 203(2):141-151(1995)
Background Reference 2:
Lin, C.S., et al. Mol. Cell. Biol. 14(4):2457-2467(1994)
Background Reference 3:
Shibata, M., et al. J. Leukoc. Biol. 54(1):10-16(1993)
Background Reference 4:
Lin, C.S., et al. J. Biol. Chem. 268(4):2781-2792(1993)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-881

List Size:
400 ul

List Price:

$495.00

Datasheet
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