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Western blot analysis in K562 cell line lysates (15ug/lane).
Western blot analysis in mouse brain tissue lysates (15ug/lane).
PMCH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PMCH using rabbit polyclonal PMCH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.
Western blot analysis in K562 cell line lysates (15ug/lane).

PMCH Antibody

CATALOG NUMBER: 61-963

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This PMCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the Central region of human PMCH.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PMCH
Additional Names:
Pro-MCH, Neuropeptide-glycine-glutamic acid, NGE, Neuropeptide G-E, Neuropeptide-glutamic acid-isoleucine, NEI, Neuropeptide E-I, Melanin-concentrating hormone, MCH, PMCH, MCH
Protein Accession Number:
P20382
Protein Gi Number:
229462960
Ncbi Gene Id Number:
5367
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The melanin-concentrating hormone (MCH) is a cyclicneuropeptide isolated initially from salmon pituitary gland andlater from rat hypothalamus (summarized by Nahon et al., 1992[PubMed 1572663]). In mammals, MCH perikarya are confined largelyto the lateral hypothalamus and zona incerta area with extensiveneuronal projections throughout the brain, including theneurohypophysis. The anatomic distribution suggests aneurotransmitter or neuromodulator role for MCH in a broad array ofneuronal functions directed toward the regulation of goal-directedbehavior, such as food intake, and general arousal. MCH and 2 otherputative neuropeptides, NEI and NGE, are encoded by the sameprecursor and appear colocalized in nerve cells and in manyinstances within the projections. The precursor is designatedpro-melanin-concentrating hormone (PMCH).
Background Reference 1:
Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010)
Background Reference 2:
Gratacos, M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 150B (6), 808-816 (2009) :
Background Reference 3:
Aziz, A., et al. Brain Pathol. 18(4):474-483(2008)
Background Reference 4:
Kokkotou, E., et al. Proc. Natl. Acad. Sci. U.S.A. 105(30):10613-10618(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-963

List Size:
400 ul

List Price:

$495.00

Datasheet
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