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46-204 (1ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-204 (1ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Pmel-17 Antibody

CATALOG NUMBER: 46-204

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CPIGENSPLLSGQQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Skin lysates, corresponding to the M-beta fragment of the precursor protein (Leonhardt et al, Mol Biol Cell. 2013 Apr;24(7):964-81.PMID: 23389629). Calculated MW of 70.3kDa according to NP_008859.1. Primary incubation was 1 hour.
Positive Control 1:
Cat. No. 1376 - Human Skin Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SILV
Additional Names:
silver homolog (mouse) , PMEL17, SILV , D12S53E, ME20, SI, SIL, gp100 , Melanocyte protein mel 17, Pmel 17, Silver, mouse, homolog of, silver (mouse homolog) like, silver (mouse homolog)-like, silver homolog
Protein Accession Number:
NP_008859.1
Protein Gi Number:
5902084
Ncbi Gene Id Number:
6490
Background Reference 1:
Fowler DM, Koulov AV, Alory-Jost C, Marks MS, Balch WE, Kelly JW. Functional amyloid formation within mammalian tissue. PLoS Biol. 2006 Jan;4(1):e6.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-204

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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