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Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

PMF1 Antibody

CATALOG NUMBER: 60-440

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PMF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-135 amino acids from the Central region of human PMF1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PMF1
Additional Names:
Polyamine-modulated factor 1, PMF-1, PMF1 {ECO:0000312, EMBL:AAH650311}
Protein Accession Number:
Q6P1K2
Protein Gi Number:
114152119
Ncbi Gene Id Number:
1.00528E+13
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Background Reference 1:
Aleman, A., et al. Clin. Cancer Res. 14(24):8236-8243(2008)
Background Reference 2:
Kline, S.L., et al. J. Cell Biol. 173(1):9-17(2006)
Background Reference 3:
Obuse, C., et al. Nat. Cell Biol. 6(11):1135-1141(2004)
Background Reference 4:
Wang, Y., et al. Biochem. J. 366 (PT 1), 79-86 (2002) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-440

List Size:
400 ul

List Price:

$495.00

Datasheet
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