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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

PMM2 Antibody

CATALOG NUMBER: 60-261

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Monkey, Bovine
Immunogen:
This PMM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the C-terminal region of human PMM2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PMM2
Additional Names:
Phosphomannomutase 2, PMM 2, PMM2
Protein Accession Number:
O15305
Ncbi Gene Id Number:
5373
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene catalyzes theisomerization of mannose 6-phosphate to mannose 1-phosphate, whichis a precursor to GDP-mannose necessary for the synthesis ofdolichol-P-oligosaccharides. Mutations in this gene have been shownto cause defects in glycoprotein biosynthesis, which manifests ascarbohydrate-deficient glycoprotein syndrome type I. [provided byRefSeq].
Background Reference 1:
Vega, A.I., et al. Hum. Mutat. 30(5):795-803(2009)
Background Reference 2:
Coman, D., et al. Am. J. Med. Genet.146(3):389-392(2008)
Background Reference 3:
Vermeer, S., et al. J. Neurol. 254(10):1356-1358(2007)
Background Reference 4:
Coman, D., et al.Clin Neurosci 14(7):668-672(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-261

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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