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Western blot analysis of extracts of various cell lines, using PMPCB antibody (19-337) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PMPCB antibody (19-337) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PMPCB Antibody

CATALOG NUMBER: 19-337

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 110-380 of human PMPCB (NP_004270.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
LO2
Positive Control 2:
MCF7
Positive Control 3:
HT-1080
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse kidney
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PMPCB
Additional Names:
Mitochondrial-processing peptidase subunit beta, Beta-MPP, P-52, PMPCB, MPPB
Ncbi Gene Id Number:
9512
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-337

List Size:
100 uL

List Price:

$436.00

Datasheet
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