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Western blot analysis of PNAS4 in EL4 cell lysate with PNAS4 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of PNAS4 in EL4 cell lysate with PNAS4 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PNAS4 Antibody

CATALOG NUMBER: 5217

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Immunogen:
PNAS4 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PNAS4.
The immunogen is located within the last 50 amino acids of PNAS4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PNAS4 antibody can be used for detection of PNAS4 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Purification:
PNAS4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PNAS4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PNAS4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DESI2
Additional Names:
PNAS4 Antibody: DESI, DESI1, DeSI-2, PNAS-4, PPPDE1, CGI-146, FAM152A, C1orf121, Desumoylating isopeptidase 2, PPPDE peptidase domain-containing protein 1
Protein Accession Number:
NP_057160
Protein Gi Number:
38708309
Ncbi Gene Id Number:
51029
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PNAS4 Antibody: PNAS4 is a highly conserved protein that shares high homology from plants to animals. It was initially identified as a putative apoptosis-related protein in the human acute promyelocytic leukemia cell line NB4. PNAS4 is activated during the early response to DNA damage and can inhibit proliferation via apoptosis when overexpressed in some tumor cells such as U2OS, SKOV3, and A549. PNAS4 inhibits tumor cell proliferation through the following mechanisms: (i) overexpression of PNAS4 causes S phase arrest by regulating the expression of cell cycle-related proteins and (ii) PNAS4 induces apoptosis through the mitochondrial apoptosis pathway. Recent evidence has shown that PNAS4 may be involved in the genesis of some cancers and could be a potential candidate for lung cancer therapy alone or in combination with gemcitabine. At least two isoforms of PNAS4 are known to exist.
Background Reference 1:
Yao S, Xie L, Qian M, et al. Pnas4 is a novel regulator for convergence and extension during vertebrate gastrulation. FEBS Lett.2008; 582:2325-32.
Background Reference 2:
Yan F, Gou L, Yang J, et al. A novel pro-apoptosis gene PNAS4 that induces apoptosis in A549 human lung adenocarcinoma cells and inhibits tumor growth in mice. Biochimie2009; 91:502-7.
Background Reference 3:
Yuan Z, Liu H, Yan F, et al. Improved therapeutic efficacy against murine carcinoma by combining honokiol with gene therapy of PNAS-4, a novel pro-apoptotic gene. Cancer Sci.2009; 100:1757-66.
Background Reference 4:
Yuan Z, Yan F, Wang YS, et al. PNAS-4, a novel pro-apoptotic gene, can potentiate antineoplastic effects of cisplatin. Cancer Chemother. Pharmacol.2009; .

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5217

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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