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Western blot analysis of extracts of various cell lines, using PNKP antibody (22-410) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (22-410) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (22-410). GreenGFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Western blot analysis of extracts of various cell lines, using PNKP antibody (22-410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PNKP Antibody

CATALOG NUMBER: 22-410

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
HL-60
Positive Control 2:
HepG2
Positive Control 3:
Rat liver
Predicted Molecular Weight:
Observed: 57kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PNKP
Additional Names:
PNKP, polynucleotide kinase 3'-phosphatase, PNK, polynucleotide kinase 3-prime-phosphatase, MCSZ, EIEE10
Ncbi Gene Id Number:
11284
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-410

List Size:
100 uL

List Price:

$436.00

Datasheet
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