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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Western blot analysis of PNKP using rabbit polyclonal PNKP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PNKP gene.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

PNKP Antibody

CATALOG NUMBER: 57-487

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PNKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the N-terminal region of human PNKP.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PNKP
Additional Names:
Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase, PNKP
Protein Accession Number:
Q96T60
Protein Gi Number:
50401132
Ncbi Gene Id Number:
11284
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This locus represents a gene involved in DNA repair. Inresponse to ionizing radiation or oxidative damage, the proteinencoded by this locus catalyzes 5' phosphorylation and 3'dephosphorylation of nucleic acids. Mutations at this locus havebeen associated with microcephaly, seizures, and developmentaldelay.
Background Reference 1:
Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Background Reference 2:
Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
Background Reference 3:
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Background Reference 4:
Shen, J., et al. Nat. Genet. 42(3):245-249(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-487

List Size:
400 ul

List Price:

$495.00

Datasheet
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