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46-206 (1ug/ml) staining of Human Kisney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-206 (1ug/ml) staining of Human Kisney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PODXL Antibody

CATALOG NUMBER: 46-206

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-DNLTKDDLDEEEDTH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 160kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 58.6kDa according to NP_001018121.1 and 55.4kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kershaw et al., J Biol Chem. 1997 Jun 20;272(25):15708-14.; PMID: 9188463). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2).
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PODXL
Additional Names:
PODXL, podocalyxin-like, Gp200, MGC138240, PCLP
Protein Accession Number:
NP_001018121.1,NP_005388.2
Protein Gi Number:
66277202
Ncbi Gene Id Number:
5420
Background Reference 1:
Nielsen JS, Graves ML, Chelliah S, Vogl AW, Roskelley CD, McNagny KM. The CD34-Related Molecule Podocalyxin Is a Potent Inducer of Microvillus Formation. PLoS ONE. 2007 Feb 21;2:e237.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-206

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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