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Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of POFUT1 in human heart tissue with POFUT antibody at 2.5 μg/mL.
Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

POFUT1 Antibody

CATALOG NUMBER: 4765

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%)
Immunogen:
POFUT1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1.
The immunogen is located within amino acids 90 - 140 of POFUT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
POFUT1 antibody can be used for the detection of POFUT1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Purification:
POFUT1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
POFUT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
POFUT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POFUT1
Additional Names:
POFUT1 Antibody: DDD2, FUT12, O-FUT, OFUCT1, O-Fuc-T, O-FucT-1, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1
Protein Accession Number:
NP_056167
Protein Gi Number:
27436889
Ncbi Gene Id Number:
23509
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POFUT1 Antibody: POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.
Background Reference 1:
Wang Y, Shao L, Shi S, et al. Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase. J. Biol. Chem.2001; 276:40338-45.
Background Reference 2:
Loriol C, Dupuy F, Rampal R, et al. Molecular evolution of protein O-fucosyltransferase genes and splice variants. Glycobiology2006; 16:736-47.
Background Reference 3:
Stahl M, Uemura K, Ge C, et al. Roles of POFUT1 and O-fucose in mammalian Notch signaling. J. Biol. Chem.2008; 283:13638-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4765

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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