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Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.

POFUT2 Antibody

CATALOG NUMBER: 26-081

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POFUT2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
POFUT2 antibody can be used for detection of POFUT2 by ELISA at 1:312500. POFUT2 antibody can be used for detection of POFUT2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
50 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store POFUT2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
POFUT2
Additional Names:
POFUT2, C21orf80, FUT13
Protein Accession Number:
NP_598368
Protein Gi Number:
34147487
Ncbi Gene Id Number:
23275
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POFUT2 catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).
Background Reference 1:
Luo, Y., (2006) J. Biol. Chem. 281 (14), 9385-9392.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-081

List Size:
100 ul

List Price:

$519.00

Datasheet
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