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Western blot analysis in HepG2 cell line lysates (35ug/lane).
POFUT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

POFUT2 Antibody

CATALOG NUMBER: 56-696

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This POFUT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the Central region of human POFUT2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POFUT2
Additional Names:
GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2, O-FucT-2, POFUT2, C21orf80, FUT13, KIAA0958
Protein Accession Number:
Q9Y2G5
Protein Gi Number:
59803123
Ncbi Gene Id Number:
23275
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Fucose is typically found as a terminal modification ofbranched chain glycoconjugates, but it also exists in directO-linkage to serine or threonine residues within cystine knotmotifs in epidermal growth factor (EGF; MIM 131530)-like repeats orthrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is anO-fucosyltransferase that use THBS type-1 repeats as substrates(Luo et al., 2006 [PubMed 16464857]).
Background Reference 1:
Feng, T., et al. Hum. Genet. 128(3):269-280(2010)
Background Reference 2:
Luo, Y., et al. J. Biol. Chem. 281(14):9385-9392(2006)
Background Reference 3:
Luo, Y., et al. J. Biol. Chem. 281(14):9393-9399(2006)
Background Reference 4:
Menzel, O., et al. Genomics 84(2):320-330(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-696

List Size:
400 ul

List Price:

$495.00

Datasheet
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