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Western blot analysis of extracts of various cell lines, using POLB antibody (16-758) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and POLB knockout (KO) HeLa cells, using POLB antibody (16-758) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using POLB antibody (16-758). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using POLB antibody (16-758) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
KO

POLB Antibody, KO Validated

CATALOG NUMBER: 16-758

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:200 - 1:1000
IF: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 37kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
POLB
Additional Names:
POLB, DNA pol beta, Mutant DNA polymerase beta, DNA polymerase beta, DNA polymerase beta subunit
Ncbi Gene Id Number:
5423
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-758

List Size:
100 uL

List Price:

$540.00

Datasheet
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