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Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of POLG Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
POLG Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).

POLG Antibody

CATALOG NUMBER: 58-118

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
140 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLG
Additional Names:
DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA
Protein Accession Number:
P54098
Protein Gi Number:
1706507
Ncbi Gene Id Number:
5428
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mitochondrial DNA polymerase is heterotrimeric, consistingof a homodimer of accessory subunits plus a catalytic subunit. Theprotein encoded by this gene is the catalytic subunit ofmitochondrial DNA polymerase. The encoded protein contains apolyglutamine tract near its N-terminus that may be polymorphic.Defects in this gene are a cause of progressive externalophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensoryataxic neuropathy dysarthria and ophthalmoparesis (SANDO),Alpers-Huttenlocher syndrome (AHS), and mitochondrialneurogastrointestinal encephalopathy syndrome (MNGIE). Twotranscript variants encoding the same protein have been found forthis gene.
Background Reference 1:
Tong, Z.B., et al. Fertil. Steril. 94(7):2932-2934(2010)
Background Reference 2:
Stewart, J.D., et al. Hepatology 52(5):1791-1796(2010)
Background Reference 3:
Batabyal, D., et al. J. Biol. Chem. 285(44):34191-34201(2010)
Background Reference 4:
Wang, W., et al. Nucleic Acids Res. (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-118

List Size:
400 ul

List Price:

$495.00

Datasheet
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