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Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.

POLK Antibody

CATALOG NUMBER: 26-347

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
POLK antibody can be used for detection of POLK by ELISA at 1:62500. POLK antibody can be used for detection of POLK by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
721_B Cell Lysate
Predicted Molecular Weight:
99 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store POLK antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
POLK
Additional Names:
POLK, DINB1, DINP, POLQ
Protein Accession Number:
NP_057302
Protein Gi Number:
7705344
Ncbi Gene Id Number:
51426
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POLK belongs to the DNA polymerase type-Y family. It contains 2 Rad18-type zinc fingers and 1 umuC domain. POLK is a DNA polymerase specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. It lacks 3'-5' proofreading exonuclease activity. POLK forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Background Reference 1:
Hile, S.E. (2008) Nucleic Acids Res. 36 (2), 688-696.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-347

List Size:
100 ul

List Price:

$519.00

Datasheet
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