Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis in 293 cell line lysates (35ug/lane).
Western blot analysis in 293 cell line lysates (35ug/lane).

POLK Antibody

CATALOG NUMBER: 60-812

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
99 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLK
Additional Names:
DNA polymerase kappa, DINB protein, DINP, POLK, DINB1
Protein Accession Number:
Q9UBT6
Protein Gi Number:
59798438
Ncbi Gene Id Number:
51426
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
External and internal DNA-damaging agents continuallythreaten the integrity of genetic material in cells. Although avariety of repair mechanisms exist to remove the resulting lesions,some lesions escape repair and block the replication machinery.Members of the Y family of DNA polymerases, such as POLK, permitthe continuity of the replication fork by allowing replicationthrough such DNA lesions. Each Y family polymerase has a uniqueDNA-damage bypass and fidelity profile. POLK is specialized for theextension step of lesion bypass (summary by Lone et al., 2007[PubMed 17317631]).
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :
Background Reference 3:
Katafuchi, A., et al. Nucleic Acids Res. 38(3):859-867(2010)
Background Reference 4:
Fukuda, H., et al. J. Biol. Chem. 284(38):25585-25592(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-812

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369