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43-290 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-290 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-290 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

polo-like kinase 3 Antibody

CATALOG NUMBER: 43-290

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: PDRLPISSCVTVPD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa (calculated MW of 71.6kDa according to NP_004064.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows vesicular staining in the cystoplasm of collective tubules. Recommended concentration, 5-10ug/ml.
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
polo-like kinase 3
Additional Names:
PLK3, polo-like kinase 3, CNK, FNK, PRK, FGF-inducible kinase, PLK-3, cytokine-inducible kinase, cytokine-inducible serine/threonine-protein kinase, proliferation-related kinase, serine/threonine-protein kinase PLK3
Protein Accession Number:
NP_004064.2
Protein Gi Number:
41872374
Ncbi Gene Id Number:
1263
Background Reference 1:
Wang J, Beauchemin M, Bertrand R.Bcl-xL phosphorylation at Ser49 by polo kinase 3 during cell cycle progression and checkpoints.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-290

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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