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Western blot analysis in Hela cell line lysates (35ug/lane).
POLR1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).

POLR1B Antibody

CATALOG NUMBER: 56-464

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human POLR1B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
128 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLR1B
Additional Names:
DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, POLR1B
Protein Accession Number:
Q9H9Y6
Protein Gi Number:
92090637
Ncbi Gene Id Number:
84172
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Eukaryotic RNA polymerase I (pol I) is responsible for thetranscription of ribosomal RNA (rRNA) genes and production of rRNA,the primary component of ribosomes. Pol I is a multisubunit enzymecomposed of 6 to 14 polypeptides, depending on the species. Most ofthe mass of the pol I complex derives from the 2 largest subunits,Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither andGrummt, 1996 [PubMed 8921381]).
Background Reference 1:
Wen, L., et al. Biochem. Biophys. Res. Commun. 367(4):846-851(2008)
Background Reference 2:
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Background Reference 3:
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Background Reference 4:
Panova, T.B., et al. Mol. Cell. Biol. 26(16):5957-5968(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-464

List Size:
400 ul

List Price:

$495.00

Datasheet
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