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Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: HepG2 whole cell lysate Lane 7: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with POLR1C antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: HepG2 whole cell lysate Lane 7: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

POLR1C Antibody

CATALOG NUMBER: 61-539

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This POLR1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-346 amino acids from the C-terminal region of human POLR1C.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLR1C
Additional Names:
DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit C, RNA polymerases I and III subunit AC1, AC40, DNA-directed RNA polymerases I and III 40 kDa polypeptide, RPA40, RPA39, RPC40, POLR1C, POLR1E
Protein Accession Number:
O15160
Ncbi Gene Id Number:
9533
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Background Reference 1:
Rush,J., Nat. Biotechnol. 23 (1), 94-101 (2005)
Background Reference 2:
Hirschler-Laszkiewicz,I., J. Biol. Chem. 278 (21), 18953-18959 (2003)
Background Reference 3:
Dammann,R., Biochim. Biophys. Acta 1396 (2), 153-157 (1998)
Background Reference 4:
Seither,P., Chromosoma 106 (4), 216-225 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-539

List Size:
400 ul

List Price:

$495.00

Datasheet
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