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Western blot analysis of extracts of various cell lines, using POLR1D antibody (23-356) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 40s.
Immunofluorescence analysis of A549 cells using POLR1D antibody (23-356). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using POLR1D antibody (23-356) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.

POLR1D Antibody

CATALOG NUMBER: 23-356

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human POLR1D (NP_057056.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
MCF7
Positive Control 3:
A-549
Positive Control 4:
SW480
Positive Control 5:
Jurkat
Predicted Molecular Weight:
Observed: 15kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
POLR1D
Additional Names:
POLR1D, FLJ20616, MGC9850, POLR1C, RPA16, RPA9, RPAC2, RPO1-3, AC19, TCS2, RPC16
Ncbi Gene Id Number:
51082
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS), a craniofacial development disorder. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-356

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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