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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

POLR1E Antibody

CATALOG NUMBER: 58-944

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This POLR1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human POLR1E.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLR1E
Additional Names:
DNA-directed RNA polymerase I subunit RPA49, RNA polymerase I subunit A49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53, POLR1E, PAF53, PRAF1
Protein Accession Number:
Q9GZS1
Protein Gi Number:
62901107
Ncbi Gene Id Number:
64425
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 3:
Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)
Background Reference 4:
Yamamoto, K., et al. Mol. Cell. Biol. 24(14):6338-6349(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-944

List Size:
400 ul

List Price:

$495.00

Datasheet
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