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Western blot analysis of extracts of various cell lines, using POLR2A antibody (13-946) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using POLR2A antibody (13-946) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using POLR2A antibody (13-946) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

POLR2A Antibody

CATALOG NUMBER: 13-946

Clonality:
Polyclonal
Tested Applications:
ChIP, IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Pig, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
ChIP: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
BT-474
Positive Control 3:
H460
Positive Control 4:
Jurkat
Positive Control 5:
Mouse testis
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 250kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
POLR2A
Additional Names:
POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1
Ncbi Gene Id Number:
5430
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-946

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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