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Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.

POLR2B Antibody

CATALOG NUMBER: 27-602

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
POLR2B antibody can be used for detection of POLR2B by ELISA at 1:1562500. POLR2B antibody can be used for detection of POLR2B by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
134 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store POLR2B antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
POLR2B
Additional Names:
POLR2B, RPB2, POL2RB, hsRPB2, hRPB140
Protein Accession Number:
NP_000929
Protein Gi Number:
4505941
Ncbi Gene Id Number:
5431
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
Background Reference 1:
Zhou, M., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (22), 12666-12671.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-602

List Size:
100 ul

List Price:

$519.00

Datasheet
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