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Western blot analysis in 293 cell line lysates (35ug/lane).
Western blot analysis in 293 cell line lysates (35ug/lane).

POLR2B Antibody

CATALOG NUMBER: 57-949

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This POLR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 797-826 amino acids from the Central region of human POLR2B.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
134 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLR2B
Additional Names:
DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II subunit 2, RNA polymerase II subunit B2, POLR2B
Protein Accession Number:
P30876
Protein Gi Number:
401012
Ncbi Gene Id Number:
5431
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the second largest subunit of RNApolymerase II, the polymerase responsible for synthesizingmessenger RNA in eukaryotes. This subunit, in combination with atleast two other polymerase subunits, forms a structure within thepolymerase that maintains contact in the active site of the enzymebetween the DNA template and the newly synthesized RNA. [providedby RefSeq].
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Background Reference 3:
Tu, S., et al. Nat. Struct. Mol. Biol. 15(4):419-421(2008)
Background Reference 4:
Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-949

List Size:
400 ul

List Price:

$495.00

Datasheet
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