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Western blot analysis of extracts of various cell lines, using POLR2C antibody (18-131) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 150ug extracts of SW620 cells using 3ug POLR2C antibody (18-131). Western blot was performed from the immunoprecipitate using POLR2C antibody (18-131) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using POLR2C antibody (18-131) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

POLR2C Antibody

CATALOG NUMBER: 18-131

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human POLR2C (NP_116558.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:20 - 1:50
Positive Control 1:
SW620
Positive Control 2:
OVCAR3
Positive Control 3:
Mouse spleen
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
POLR2C
Additional Names:
RPB3, RPB31, hRPB33, hsRPB3, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB33, polymerase (RNA) II (DNA directed) polypeptide C, 33kDa, polymerase (RNA) II subunit C
Ncbi Gene Id Number:
5432
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-131

List Size:
100 uL

List Price:

$436.00

Datasheet
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