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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
Western blot analysis of POLR2D using rabbit polyclonal POLR2D Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

POLR2D Antibody

CATALOG NUMBER: 59-030

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This POLR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the Central region of human POLR2D.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLR2D
Additional Names:
DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D
Protein Accession Number:
O15514
Ncbi Gene Id Number:
5433
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the fourth largest subunit of RNApolymerase II, the polymerase responsible for synthesizingmessenger RNA in eukaryotes. In yeast, this polymerase subunit isassociated with the polymerase under suboptimal growth conditionsand may have a stress protective role. A sequence for a ribosomalpseudogene is contained within the 3' untranslated region of thetranscript from this gene.
Background Reference 1:
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Background Reference 2:
Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005)
Background Reference 3:
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)
Background Reference 4:
Kaehlcke, K., et al. Mol. Cell 12(1):167-176(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-030

List Size:
400 ul

List Price:

$495.00

Datasheet
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