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Western blot analysis of extracts of various cell lines, using POLR2G antibody (22-504) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using POLR2G antibody (22-504) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

POLR2G Antibody

CATALOG NUMBER: 22-504

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human POLR2G (NP_002687.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
U-251MG
Positive Control 2:
MCF-7
Positive Control 3:
SW620
Positive Control 4:
A-549
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 20kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
POLR2G
Additional Names:
RNA polymerase II seventh subunit, DNA directed RNA polymerase II polypeptide G, DNA directed RNA polymerase II 19 kda polypeptide, hsRPB7, hRPB19, RPB7, MGC138369, MGC138367, polymerase (RNA) II (DNA directed) polypeptide G, POLR2G
Ncbi Gene Id Number:
5436
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-504

List Size:
100 uL

List Price:

$436.00

Datasheet
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