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Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Antibody used in WB on Human Heart at 0.2-1 ug/ml.

POLR3B Antibody

CATALOG NUMBER: 30-455

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Drosophila, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Drosophila, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
POLR3B antibody can be used for detection of POLR3B by ELISA at 1:12500. POLR3B antibody can be used for detection of POLR3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Predicted Molecular Weight:
128 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store POLR3B antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
POLR3B
Additional Names:
POLR3B, FLJ10388, RPC2, C128, HLD8
Protein Accession Number:
NP_060552
Protein Gi Number:
238908503
Ncbi Gene Id Number:
55703
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Background Reference 1:
Yee, N.S., PLoS Biol. 5 (11), E312 (2007).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-455

List Size:
100 ul

List Price:

$519.00

Datasheet
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