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Western blot analysis of POLR3F in human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of POLR3F in human brain tissue with POLR3F antibody at 2.5 μg/mL.
Immunofluorescence of POLR3F in human brain tissue with POLR3F antibody at 20 μg/mL.
Western blot analysis of POLR3F in human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

POLR3F Antibody

CATALOG NUMBER: 4727

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
POLR3F antibody was raised against a 21 amino acid synthetic peptide from near the amino terminus of human POLR3F.
The immunogen is located within the first 50 amino acids of POLR3F.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
POLR3F Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
POLR3F Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
POLR3F antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLR3F
Additional Names:
POLR3F Antibody: RPC6, RPC39, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F, RNA polymerase III subunit C6
Protein Accession Number:
CAC11110
Protein Gi Number:
10636565
Ncbi Gene Id Number:
10621
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POLR3F Antibody: The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.
Background Reference 1:
Hu P, Wu S, Sun Y, et al. Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. Mol. Cell Biol.2002; 22:8044-55.
Background Reference 2:
Haeusler RA and Engelke DR. Spatial organization of transcription by RNA polymerase III. Nucleic Acids Res.2006; 34:4826-36. Epub 2006 Sep 13.
Background Reference 3:
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4727

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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