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Western blot analysis of extracts of various cell lines, using POLRMT antibody (16-132) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug POLRMT antibody (16-132). Western blot was performed from the immunoprecipitate using POLRMT antibody (16-132) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using POLRMT antibody (16-132) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

POLRMT Antibody

CATALOG NUMBER: 16-132

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
HepG2
Positive Control 3:
293T
Predicted Molecular Weight:
Observed: 139kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
POLRMT
Additional Names:
POLRMT, polymerase (RNA) mitochondrial (DNA directed), APOLMT, MTRNAP, MTRPOL, h-mtRPOL, DNA-directed RNA polymerase, mitochondrial
Ncbi Gene Id Number:
5442
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-132

List Size:
100 uL

List Price:

$436.00

Datasheet
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