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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br>Loading: 15 μg of lysate. Antibodies: POLRMT, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Immunofluorescence Validation of POLRMT in Daudi Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 3 Immunofluorescence Validation of POLRMT in Mouse Brain</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 4 Immunofluorescence Validation of POLRMT in Rat Brain</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 WB Validation in Human Cell Lines
Loading: 15 μg of lysate. Antibodies: POLRMT, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

POLRMT (CT) Antibody

CATALOG NUMBER: 9561

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Cow (82%); Chimpanzee (100%) ; monkey (82%); Cat (90%)
Immunogen:
Anti-POLRMT antibody (9561) was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human POLRMT.
The immunogen is located within the last 50 amino acids of POLRMT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WB: 2 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight:
Predicted: 139kD
Observed: 139 kD
Isoforms:
Human POLRMT has 1 isoform (1230aa, 138.6kD). Mouse POLRMT has one isoform (1207aa, 136.7kD). Rat POLRMT has one isoform (1203aa, 135.8kD). 9561 can detect POLRMT in human, mouse and rat.
Purification:
POLRMT Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
POLRMT Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
POLRMT antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POLRMT
Additional Names:
DNA-directed RNA polymerase, mitochondrial, MtRPOL, POLRMT Isoforms
Protein Accession Number:
O0411.2
Protein Gi Number:
50403691
Ncbi Gene Id Number:
5442
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POLRMT Antibody: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. POLRMT mutations impair mitochondrial transcription causing neurological disease. Knock-down of MRPL12 by RNA interference results in instability of POLRMT.
Background Reference 1:
Inatomi et al. Biochim Biophys Acta Mol Cell Res. 2022; 1869(1); 119167
Background Reference 2:
Olahova et al. Nat Commun. 2021; 12(1):1135
Background Reference 3:
Nouws et al. J Biol Chem 2016; 291(2):989-997

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9561

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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