POMC Antibody

CATALOG NUMBER: 46-208

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-NAIIKNAYKKGE
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 27kDa band observed in lysates of cell line NIH3T3 and approx 30kDa in Rat Brain lysates (calculated MW of 26.7kDa according to Mouse NP_001265510.1 1 and 26.6kDa according to Rat NP_647542.1). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration: 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
POMC
Additional Names:
POMC, proopiomelanocortin, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating, hormone/ beta-endorphin, LPH, NPP, OTTHUMP00000119991, OTTHUMP00000200964, adrenocorticotropic hormone, adrenocorticotropin, alpha-MSH, alpha-melanocyte-stimulating hormone, beta-LPH, beta-endorphin, beta-melanocyte-stimulating hormone, corticotropin-like intermediary peptide, gamma-LPH, gamma-MSH, lipotropin beta, lipotropin gamma, melanotropin alpha, melanotropin beta, melanotropin gamma, met-enkephalin, pro-ACTH-endorphin, proopiomelanocortin preproprotein
Protein Accession Number:
NP_000930.1,NP_001030333.1
Protein Gi Number:
4505949
Ncbi Gene Id Number:
5443
Background Reference 1:
Muhlhausler BS, Adam CL, Marrocco E, Findlay PA, Roberts CT, McFarlane JR, Kauter KG, McMillen IC. Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation. J Physiol. 2005 Jan 20;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-208

List Size:
0.1 mg

List Price:

$495.00

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