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POMT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in ZR-75-1,K562 cell line lysates (35ug/lane).
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
POMT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

POMT1 Antibody

CATALOG NUMBER: 56-386

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This POMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-735 amino acids from the C-terminal region of human POMT1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
85 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POMT1
Additional Names:
Protein O-mannosyl-transferase 1, Dolichyl-phosphate-mannose--protein mannosyltransferase 1, POMT1
Protein Accession Number:
Q9Y6A1
Protein Gi Number:
332278226
Ncbi Gene Id Number:
10585
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is anO-mannosyltransferase that requires interaction with the product ofthe POMT2 gene for enzymatic function. The encoded protein is foundin the membrane of the endoplasmic reticulum. Defects in this geneare a cause of Walker-Warburg syndrome (WWS) and limb-girdlemuscular dystrophy type 2K (LGMD2K). Several transcript variantsencoding different isoforms have been found for this gene.
Background Reference 1:
Manya, H., et al. J. Biochem. 147(3):337-344(2010)
Background Reference 2:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 3:
Cotarelo, R.P., et al. Clin. Genet. 76(1):108-112(2009)
Background Reference 4:
Mercuri, E., et al. Neurology 72(21):1802-1809(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-386

List Size:
400 ul

List Price:

$495.00

Datasheet
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