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42-513 (0.2ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-513 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-513 (0.2ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PON2 Antibody

CATALOG NUMBER: 42-513

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Dog
Immunogen:
The immunogen for this antibody is: C-EKPRARELRIS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa double band observed in lysates of HepG2 (calculated MW of 39.4kDa according to NP_000296.2 and of 38.0kDa according to NP_001018171.1). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour.An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2; NP_001018171.1).
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PON2
Additional Names:
PON2, paraoxonase 2, A-esterase 2, aromatic esterase 2, paraoxonase nirs, serum aryldialkylphosphatase 2, serum paraoxonase/arylesterase 2
Protein Accession Number:
NP_000296.2,NP_001018171.1
Protein Gi Number:
66529294
Ncbi Gene Id Number:
5445

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-513

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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