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Western blot analysis in MCF-7(lane 1),A375(lane 2) cell line lysates (35ug/lane).
POSTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of POSTN Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in MCF-7(lane 1),A375(lane 2) cell line lysates (35ug/lane).

POSTN Antibody

CATALOG NUMBER: 56-145

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This POSTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 687-716 amino acids from the C-terminal region of human POSTN.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
93 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POSTN
Additional Names:
Periostin, PN, Osteoblast-specific factor 2, OSF-2, POSTN, OSF2
Protein Accession Number:
Q15063
Protein Gi Number:
93138709
Ncbi Gene Id Number:
10631
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POSTN binds to heparin. It induces cell attachment and spreading and plays a role in cell adhesion and may play a role in extracellular matrix mineralization.
Background Reference 1:
Zhu, M., et al. Gynecol. Oncol. 119(2):337-344(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Takada, M., et al. Biochem. Biophys. Res. Commun. 399(2):221-226(2010)
Background Reference 4:
Sidhu, S.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(32):14170-14175(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-145

List Size:
400 ul

List Price:

$495.00

Datasheet
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