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Antibody used in WB on Human Jurkat 1 ug/ml.
Antibody used in WB on Human Jurkat 1 ug/ml.

POU1F1 Antibody

CATALOG NUMBER: 27-336

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU1F1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
POU1F1 antibody can be used for detection of POU1F1 by ELISA at 1:312500. POU1F1 antibody can be used for detection of POU1F1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
33 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store POU1F1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
POU1F1
Additional Names:
POU1F1, PIT1, CPHD1, GHF-1, Pit-1, POU1F1a
Protein Accession Number:
NP_000297
Protein Gi Number:
4505955
Ncbi Gene Id Number:
5449
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.
Background Reference 1:
Dattani, M.T. et al., (2003) Growth Horm. IGF Res. 16 (9), 1207-1209.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-336

List Size:
100 ul

List Price:

$473.00

Datasheet
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