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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with 43-149 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 43-149 (1mg/ml) in Lane B. Prim
43-149 (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with 43-149 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 43-149 (1mg/ml) in Lane B. Prim

POU2AF1 Antibody

CATALOG NUMBER: 43-149

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: YTEYVPHEAVSCP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. The 38kDa was also observed in cell line Daudi. Recommended concentration, 0.3-1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
POU2AF1
Additional Names:
POU2AF1, POU class 2 associating factor 1, BOB1, OBF-1, OBF1, OCAB, POU domain, class 2, associating factor 1
Protein Accession Number:
NP_006226.2
Protein Gi Number:
167900478
Ncbi Gene Id Number:
5450
Background Reference 1:
Duan H, Xiang H, Ma L, Boxer LM, Functional long-range interactions of the IgH 3' enhancers with the bcl-2 promoter region in t(14;18) lymphoma cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-149

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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