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45-116 (0.3ug/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human BOB1 with DYKDDDDK tag probed with 45-116(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 45-116(1mg/ml) in Lane B. Primary inc
45-116 (0.3ug/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

POU2AF1 Antibody

CATALOG NUMBER: 45-116

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DAYALNHTLSVEGF
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 30kDa band seen in mouse spleen lysate (Predicted MW of 28kDa according to NP_006226). Recommended for use at 1-3ug/ml.
Positive Control 1:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
POU2AF1
Additional Names:
BOB1, OBF1, POU2AF1, OBF-1, POU domain, class 2, associating factor 1, POU class 2 associating factor 1, OCAB
Protein Accession Number:
NP_006226.2
Protein Gi Number:
167900478
Ncbi Gene Id Number:
5450
Background Reference 1:
Strubin M, Newell JW, Matthias P. OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins. Cell. 1995 Feb 10;80(3):497-506.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-116

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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