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Western blot analysis in HL-60 cell line lysates (35ug/lane).
POU2F1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

POU2F1 Antibody

CATALOG NUMBER: 55-997

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Pig, Rat, Xenopus
Immunogen:
This POU2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-391 amino acids from the Central region of human POU2F1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
76 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POU2F1
Additional Names:
POU domain, class 2, transcription factor 1, NF-A1, Octamer-binding protein 1, Oct-1, Octamer-binding transcription factor 1, OTF-1, POU2F1, OCT1, OTF1
Protein Accession Number:
P14859
Protein Gi Number:
28202257
Ncbi Gene Id Number:
5451
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The OCT1 transcription factor was among the firstidentified members of the POU transcription factor family(summarized by Sturm et al., 1993 [PubMed 8314572]). Members ofthis family contain the POU domain, a 160-amino acid regionnecessary for DNA binding to the octameric sequenceATGCAAAT.
Background Reference 1:
Vuzman, D., et al. Biophys. J. 99(4):1202-1211(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 4:
Laurent, B., et al. Blood 115(3):687-695(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-997

List Size:
400 ul

List Price:

$495.00

Datasheet
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