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43-195 (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-195 (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

POU2F2 / OCT2 Antibody

CATALOG NUMBER: 43-195

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: KQGLDSPSEHTDTER-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Tonsil and Mouse Thymus lysates (calculated MW of 49.5kDa according to NP_002689.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pelis et al, Am J Physiol Renal Physiol. 2006 May;290(5):F1118-26. PMID: 16368738). Recommended concentration: 0.1-0.3ug/ml.
Predicted Molecular Weight:
Approx 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
POU2F2 / OCT2
Additional Names:
POU2F2, POU class 2 homeobox 2, OCT2, OTF2, Oct-2, POU domain, class 2, transcription factor 2, homeobox protein
Protein Accession Number:
NP_002689.1
Protein Gi Number:
4505959
Ncbi Gene Id Number:
5452
Background Reference 1:
Duan H, Xiang H, Ma L, Boxer LM, Functional long-range interactions of the IgH 3' enhancers with the bcl-2 promoter region in t(14;18) lymphoma cells.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-195

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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