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Western blot analysis of POU2F3 in SK-N-SH cell lysate with POU2F3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of POU2F3 in rat brain tissue with POU2F3 antibody at 5 μg/mL.
Immunofluorescence of POU2F3 in rat brain tissue with POU2F3 antibody at 20 μg/mL.
Western blot analysis of POU2F3 in SK-N-SH cell lysate with POU2F3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

POU2F3 Antibody

CATALOG NUMBER: 7795

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
POU2F3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human POU2F3.
The immunogen is located within amino acids 370 - 420 of POU2F3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Predicted Molecular Weight:
Predicted: 48 kDa
Observed: 55 kDa
Purification:
POU2F3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
POU2F3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
POU2F3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
POU2F3
Additional Names:
POU2F3 Antibody: PLA1, OCT11, PLA-1, Epoc-1, OCT-11, OTF-11, Skn-1a, OTF11, PLA1, POU domain, class 2, transcription factor 3, Octamer-binding protein 11, 41923
Protein Accession Number:
NP_001231611
Protein Gi Number:
347658964
Ncbi Gene Id Number:
25833
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
POU2F3, also known as Epoc-1, is a member of a family of POU domain family of transcription factors (1). POU2F3 is expressed primarily in the epidermis and plays a critical role in keratinocyte proliferation and differentiation (1,2). It is a crucial transcription factor that is required for the development of sweet, umami, and bitter, but not sour taste receptor cells (3). POU2F3 is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer (4).
Background Reference 1:
Yukawa K, Yasui T, Yamamoto A, et al. Epoc-1: a POU-domain gene expressed in murine epidermal basal cells and thymic stromal cells. Gene 1993; 133:163-9.
Background Reference 2:
Cabral A, Fischer DF, Vermeij WP, et al. Distinct functional interactions of human Skn-1 isoforms with Ese-1 during keratinocyte terminal differentiation. J. Biol. Chem. 2003; 278:17792-9.
Background Reference 3:
Matsumoto I, Ohmoto M, Narukawa M, et al. Skn-1a/Pou2f3 specifies taste receptor cell lineage. Nat. Neurosci. 2011; 14:685-7.
Background Reference 4:
Zhang Z, Huettner PC, Nguyen L, et al. Aberrant promoter methylation and silencing of the POU2F3 gene in cervical cancer. Oncogene 2006; 25:5436-45.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7795

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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