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42-897 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-897 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

POU3F1 Antibody

CATALOG NUMBER: 42-897

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HEDGHEAQLEPSP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 48kDa band observed in lysates of cell line A549 (calculated MW of 45.5kDa according to NP_002690.3). Recommended concentration: 1-3ug/ml.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Predicted Molecular Weight:
Approx 48 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
POU3F1
Additional Names:
POU3F1, POU class 3 homeobox 1, OCT6, OTF6, SCIP, OTF-6, POU domain transcription factor SCIP, POU domain, class 3, transcription factor 1, octamer-binding protein 6, octamer-binding transcription factor 6
Protein Accession Number:
NP_002690.3
Protein Gi Number:
110624765
Ncbi Gene Id Number:
5453

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-897

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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