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POU4F2 Antibody

CATALOG NUMBER: 43-239

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: HGLPSHMGCMSDVD
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 70kDa band in Rat Retina lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.2kDa according to NP_599182.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
Specificity:
This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
POU4F2
Additional Names:
POU4F2, POU class 4 homeobox 2, BRN3.2, BRN3B, Brn-3b, Brn3b POU domain transcription factor, POU domain class 4 transcription factor 2, POU domain protein, POU domain, class 4, transcription factor 2
Protein Accession Number:
NP_004566.2
Protein Gi Number:
110347455
Ncbi Gene Id Number:
5458
Background Reference 1:
Qiu F, Jiang H, Xiang M, A comprehensive negative regulatory program controlled by Brn3b to ensure ganglion cell specification from multipotential retinal precursors.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-239

List Size:
0.1 mg

List Price:

$468.00

Datasheet
Shipping Info

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