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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

POU6F1 Antibody

CATALOG NUMBER: 58-483

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This POU6F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human POU6F1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POU6F1
Additional Names:
POU domain, class 6, transcription factor 1, Brain-specific homeobox/POU domain protein 5, Brain-5, Brn-5, mPOU homeobox protein, POU6F1, BRN5, MPOU, TCFB1
Protein Accession Number:
Q14863
Protein Gi Number:
2495306
Ncbi Gene Id Number:
5463
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3') (By similarity).
Background Reference 1:
Suzuki, N., et al. Int. J. Gynecol. Cancer 20(2):212-219(2010)
Background Reference 2:
Yoshioka, N., et al. Hum. Cell 22(4):94-100(2009)
Background Reference 3:
Pereira, J.H., et al. J. Struct. Biol. 167(2):159-165(2009)
Background Reference 4:
Pereira, J.H., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 64 (PT 3), 175-178 (2008) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-483

List Size:
400 ul

List Price:

$495.00

Datasheet
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