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Western blot analysis of anti-PPAP2C Antibody in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-PPAP2C Antibody in NCI-H460 cell line lysates (35ug/lane).

PPAP2C Antibody

CATALOG NUMBER: 61-512

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This PPAP2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-254 amino acids from the C-terminal region of human PPAP2C.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPAP2C
Additional Names:
Lipid phosphate phosphohydrolase 2, PAP2-gamma, PAP2-G, Phosphatidate phosphohydrolase type 2c, Phosphatidic acid phosphatase 2c, PAP-2c, PAP2c, PPAP2C, LPP2
Protein Accession Number:
O43688
Ncbi Gene Id Number:
8612
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PPAP2C is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site. This protein has been shown to possess membrane associated PAP activity.
Background Reference 1:
Long,J.S., Biochem. J. 411 (2), 371-377 (2008)
Background Reference 2:
Morris,K.E., J. Biol. Chem. 281 (14), 9297-9306 (2006)
Background Reference 3:
Nanjundan,M., Am. J. Physiol. Lung Cell Mol. Physiol. 284 (1), L1-L23 (2003)
Background Reference 4:
Zhang,N., Genesis 27 (4), 137-140 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-512

List Size:
400 ul

List Price:

$495.00

Datasheet
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