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Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of PPAPDC1A in human brain tissue with PPAPDC1A antibody at 20 μg/mL.
Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PPAPDC1A Antibody

CATALOG NUMBER: 5513

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PPAPDC1A antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PPAPDC1A.
The immunogen is located within the last 50 amino acids of PPAPDC1A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
PPAPDC1A antibody can be used for detection of PPAPDC1A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
PPAPDC1A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PPAPDC1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PPAPDC1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPAPDC1A
Additional Names:
PPAPDC1A Antibody: DPPL2, PPAPDC1, DPPL2, Phosphatidate phosphatase PPAPDC1A, Phosphatidic acid phosphatase type 2 domain-containing protein 1A
Protein Accession Number:
NP_001025230
Protein Gi Number:
73611920
Ncbi Gene Id Number:
196051
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PPAPDC1A Antibody: Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide(NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. PPAPDC1A is preferentially expressed in endothelial cells. Studies of PPAPDC1A and PAP activity suggest that they may play a role in angiogenesis.
Background Reference 1:
Takeuchi M, Harigai M, Momohara S, et al. Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase. Gene2007; 399:174-80.
Background Reference 2:
Bernard-Pierrot I, Gruel N, Stransky N, et al. Characterization of the recurrent 8p11-12 amplicon identifies PPAPDC1B, a phosphatase protein, as a new therapeutic target in breast cancer. Cancer Res.2008; 68:7165-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5513

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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