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Western blot analysis of PPAPDC1B in EL4 cell lysate with PPAPDC1B antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of PPAPDC1B in EL4 cell lysate with PPAPDC1B antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PPAPDC1B Antibody

CATALOG NUMBER: 5515

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
PPAPDC1B antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human PPAPDC1B.
The immunogen is located within amino acids 30 - 80 of PPAPDC1B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PPAPDC1B antibody can be used for detection of PPAPDC1B by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Purification:
PPAPDC1B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PPAPDC1B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PPAPDC1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPAPDC1B
Additional Names:
PPAPDC1B Antibody: DPPL1, HTPAP, DPPL1, Phosphatidate phosphatase PPAPDC1B, Phosphatidic acid phosphatase type 2 domain-containing protein 1B
Protein Accession Number:
NP_001096029
Protein Gi Number:
156523237
Ncbi Gene Id Number:
84513
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PPAPDC1B Antibody: Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide (NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. Knockdown experiments indicated that this protein is involved with multiple cell signaling pathways, including the JAK-Stat3, MAP kinase, and PKC pathways. PPAPDC1B may also potentiate the estrogen receptor pathway by down-regulating DUSP22.
Background Reference 1:
Takeuchi M, Harigai M, Momohara S, et al. Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase. Gene2007; 399:174-80.
Background Reference 2:
Bernard-Pierrot I, Gruel N, Stransky N, et al. Characterization of the recurrent 8p11-12 amplicon identifies PPAPDC1B, a phosphatase protein, as a new therapeutic target in breast cancer. Cancer Res.2008; 68:7165-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5515

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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