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Western blot analysis of PPAPDC3 in mouse heart tissue lysate with PPAPDC3 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of PPAPDC3 in mouse heart tissue with PPAPDC3 antibody at 20 μg/mL.
Western blot analysis of PPAPDC3 in mouse heart tissue lysate with PPAPDC3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PPAPDC3 Antibody

CATALOG NUMBER: 5519

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PPAPDC3 antibody was raised against a 19 amino acid synthetic peptide from near the amino of terminus human PPAPDC3.
The immunogen is located within the first 50 amino acids of PPAPDC3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
PPAPDC3 antibody can be used for detection of PPAPDC3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
PPAPDC3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PPAPDC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PPAPDC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPAPDC3
Additional Names:
PPAPDC3 Antibody: NET39, C9orf67, Probable lipid phosphate phosphatase PPAPDC3, Phosphatidic acid phosphatase type 2 domain-containing protein 3
Protein Accession Number:
EAW87966
Protein Gi Number:
119608372
Ncbi Gene Id Number:
84814
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PPAPDC3 Antibody: PPAPDC3, also known as nuclear envelope transmembrane protein 39 (NET39), was initially discovered in an in silico screen for secreted or membrane proteins. It is a member of the PAP2 superfamily of phosphatases and haloperoxidases. PPAPDC3 has recently been shown to act as a negative regulator of myoblast differentiation by diminishing the activity of the mammalian target of rapamycin TOR. PPAPDC3 is highly expressed in cardiac and skeletal muscle and becomes strongly upregulated during cultured myoblast differentiation tissues. Overexpression of PPAPDC3 in myoblasts repressed myogenesis while knockdown by RNA interference promoted differentiation indicating its part in the regulatory mechanism for myogenesis.
Background Reference 1:
Otsuki T, Ota T, Nishikawa T, et al. Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res.2005; 12:117-26.
Background Reference 2:
Liu GH, Guan T, Datta K, et al. Regulation of myoblast differentiation by the nuclear envelope protein NET39. Mol. Cell Biol.2009; 29:5800-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5519

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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