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45-118 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-118 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PPARD Antibody

CATALOG NUMBER: 45-118

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: CHPLLQEIYKDMY
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in T cell line MOLT4 lysates (calculated MW of 49.9kDa according to NP_006229.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PPARD
Additional Names:
PPARD, FAAR, NUC1, NUCI, NR1C2, NUCII, PPARB, MGC3931, PPAR-beta, peroxisome proliferative activated receptor, delta, nuclear hormone receptor 1, peroxisome proliferative activated receptor delta, OTTHUMP00000016256, OTTHUMP00000016257
Protein Accession Number:
NP_006229.1
Protein Gi Number:
5453940
Ncbi Gene Id Number:
5467
Background Reference 1:
Park BH, Vogelstein B, Kinzler KW. Genetic disruption of PPARdelta decreases the tumorigenicity of human colon cancer cells. Proc Natl Acad Sci USA. 2001 Feb 27;98(5):2598-603.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-118

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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